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genes for reca-2a-mcherry and sgrna containing ms2-binding aptamers (sgrna-ms2)  (GenScript corporation)

 
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    Structured Review

    GenScript corporation genes for reca-2a-mcherry and sgrna containing ms2-binding aptamers (sgrna-ms2)
    ( A ) Schematic of Cas9- or Cas9/RecA-mediated HDR gene editing. Cas9-mediated HDR required sgRNA, spCas9, and ssDNA donors (dot line frame). Cas9/RecA-mediated HDR required sgRNA, spCas9, RecA, and ssDNA donors (solid line frame). RecA was enriched near sgRNA via the <t>MS2-coated</t> protein. ( B ) Experimental scheme for gene editing by NHEJ or HDR in the BFP HEK293FT line. ( C ) Representative FACS analysis of HEK293FT cells from the control (Cas9 without sgRNA group). ( D ) Representative FACS analysis of HEK293FT cells treated in the Cas9 group, with Cas9, sgRNA, and ssDNA donor. ( E ) Representative FACS analysis of HEK293FT cells treated in the Cas9/RecA group, with Cas9, sgRNA, ssDNA donor, and RecA. ( F ) Quantification results of Cas9-mediated HDR and NHEJ efficiencies and Cas9/RecA-mediated HDR and NHEJ efficiencies. n = 7. * P < 0.05 and ** P < 0.01, paired Student’s t test. n.s., no significance.
    Genes For Reca 2a Mcherry And Sgrna Containing Ms2 Binding Aptamers (Sgrna Ms2), supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/genes+for+reca-2a-mcherry+and+sgrna+containing+ms2-binding+aptamers+(sgrna-ms2)/genes+for+reca+2a+mcherry+and+sgrna+containing+ms2+binding+aptamers++sgrna+ms2+/pmc06469935-167-7-12
    Average 90 stars, based on 1 article reviews
    genes for reca-2a-mcherry and sgrna containing ms2-binding aptamers (sgrna-ms2) - by Bioz Stars, 2026-11
    90/100 stars

    Images

    1) Product Images from "In vivo genome editing rescues photoreceptor degeneration via a Cas9/RecA-mediated homology-directed repair pathway"

    Article Title: In vivo genome editing rescues photoreceptor degeneration via a Cas9/RecA-mediated homology-directed repair pathway

    Journal: Science Advances

    doi: 10.1126/sciadv.aav3335

    ( A ) Schematic of Cas9- or Cas9/RecA-mediated HDR gene editing. Cas9-mediated HDR required sgRNA, spCas9, and ssDNA donors (dot line frame). Cas9/RecA-mediated HDR required sgRNA, spCas9, RecA, and ssDNA donors (solid line frame). RecA was enriched near sgRNA via the MS2-coated protein. ( B ) Experimental scheme for gene editing by NHEJ or HDR in the BFP HEK293FT line. ( C ) Representative FACS analysis of HEK293FT cells from the control (Cas9 without sgRNA group). ( D ) Representative FACS analysis of HEK293FT cells treated in the Cas9 group, with Cas9, sgRNA, and ssDNA donor. ( E ) Representative FACS analysis of HEK293FT cells treated in the Cas9/RecA group, with Cas9, sgRNA, ssDNA donor, and RecA. ( F ) Quantification results of Cas9-mediated HDR and NHEJ efficiencies and Cas9/RecA-mediated HDR and NHEJ efficiencies. n = 7. * P < 0.05 and ** P < 0.01, paired Student’s t test. n.s., no significance.
    Figure Legend Snippet: ( A ) Schematic of Cas9- or Cas9/RecA-mediated HDR gene editing. Cas9-mediated HDR required sgRNA, spCas9, and ssDNA donors (dot line frame). Cas9/RecA-mediated HDR required sgRNA, spCas9, RecA, and ssDNA donors (solid line frame). RecA was enriched near sgRNA via the MS2-coated protein. ( B ) Experimental scheme for gene editing by NHEJ or HDR in the BFP HEK293FT line. ( C ) Representative FACS analysis of HEK293FT cells from the control (Cas9 without sgRNA group). ( D ) Representative FACS analysis of HEK293FT cells treated in the Cas9 group, with Cas9, sgRNA, and ssDNA donor. ( E ) Representative FACS analysis of HEK293FT cells treated in the Cas9/RecA group, with Cas9, sgRNA, ssDNA donor, and RecA. ( F ) Quantification results of Cas9-mediated HDR and NHEJ efficiencies and Cas9/RecA-mediated HDR and NHEJ efficiencies. n = 7. * P < 0.05 and ** P < 0.01, paired Student’s t test. n.s., no significance.

    Techniques Used: Control



    Similar Products

    genes for reca-2a-mcherry and sgrna containing ms2-binding aptamers (sgrna-ms2)  (GenScript corporation)
    90
    GenScript corporation genes for reca-2a-mcherry and sgrna containing ms2-binding aptamers (sgrna-ms2)
    ( A ) Schematic of Cas9- or Cas9/RecA-mediated HDR gene editing. Cas9-mediated HDR required sgRNA, spCas9, and ssDNA donors (dot line frame). Cas9/RecA-mediated HDR required sgRNA, spCas9, RecA, and ssDNA donors (solid line frame). RecA was enriched near sgRNA via the <t>MS2-coated</t> protein. ( B ) Experimental scheme for gene editing by NHEJ or HDR in the BFP HEK293FT line. ( C ) Representative FACS analysis of HEK293FT cells from the control (Cas9 without sgRNA group). ( D ) Representative FACS analysis of HEK293FT cells treated in the Cas9 group, with Cas9, sgRNA, and ssDNA donor. ( E ) Representative FACS analysis of HEK293FT cells treated in the Cas9/RecA group, with Cas9, sgRNA, ssDNA donor, and RecA. ( F ) Quantification results of Cas9-mediated HDR and NHEJ efficiencies and Cas9/RecA-mediated HDR and NHEJ efficiencies. n = 7. * P < 0.05 and ** P < 0.01, paired Student’s t test. n.s., no significance.
    Genes For Reca 2a Mcherry And Sgrna Containing Ms2 Binding Aptamers (Sgrna Ms2), supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/genes+for+reca-2a-mcherry+and+sgrna+containing+ms2-binding+aptamers+(sgrna-ms2)/genes+for+reca+2a+mcherry+and+sgrna+containing+ms2+binding+aptamers++sgrna+ms2+/pmc06469935-167-7-12
    Average 90 stars, based on 1 article reviews
    genes for reca-2a-mcherry and sgrna containing ms2-binding aptamers (sgrna-ms2) - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    ( A ) Schematic of Cas9- or Cas9/RecA-mediated HDR gene editing. Cas9-mediated HDR required sgRNA, spCas9, and ssDNA donors (dot line frame). Cas9/RecA-mediated HDR required sgRNA, spCas9, RecA, and ssDNA donors (solid line frame). RecA was enriched near sgRNA via the MS2-coated protein. ( B ) Experimental scheme for gene editing by NHEJ or HDR in the BFP HEK293FT line. ( C ) Representative FACS analysis of HEK293FT cells from the control (Cas9 without sgRNA group). ( D ) Representative FACS analysis of HEK293FT cells treated in the Cas9 group, with Cas9, sgRNA, and ssDNA donor. ( E ) Representative FACS analysis of HEK293FT cells treated in the Cas9/RecA group, with Cas9, sgRNA, ssDNA donor, and RecA. ( F ) Quantification results of Cas9-mediated HDR and NHEJ efficiencies and Cas9/RecA-mediated HDR and NHEJ efficiencies. n = 7. * P < 0.05 and ** P < 0.01, paired Student’s t test. n.s., no significance.

    Journal: Science Advances

    Article Title: In vivo genome editing rescues photoreceptor degeneration via a Cas9/RecA-mediated homology-directed repair pathway

    doi: 10.1126/sciadv.aav3335

    Figure Lengend Snippet: ( A ) Schematic of Cas9- or Cas9/RecA-mediated HDR gene editing. Cas9-mediated HDR required sgRNA, spCas9, and ssDNA donors (dot line frame). Cas9/RecA-mediated HDR required sgRNA, spCas9, RecA, and ssDNA donors (solid line frame). RecA was enriched near sgRNA via the MS2-coated protein. ( B ) Experimental scheme for gene editing by NHEJ or HDR in the BFP HEK293FT line. ( C ) Representative FACS analysis of HEK293FT cells from the control (Cas9 without sgRNA group). ( D ) Representative FACS analysis of HEK293FT cells treated in the Cas9 group, with Cas9, sgRNA, and ssDNA donor. ( E ) Representative FACS analysis of HEK293FT cells treated in the Cas9/RecA group, with Cas9, sgRNA, ssDNA donor, and RecA. ( F ) Quantification results of Cas9-mediated HDR and NHEJ efficiencies and Cas9/RecA-mediated HDR and NHEJ efficiencies. n = 7. * P < 0.05 and ** P < 0.01, paired Student’s t test. n.s., no significance.

    Article Snippet: Genes for RecA-2a-mCherry and sgRNA containing MS2-binding aptamers (sgRNA-ms2) were synthesized in GenScript.

    Techniques: Control